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ATCC s uberis strain 0140j

S. uberis SUB1154 protein is involved in the production of IL-1β from BMMOs. Bovine mammary macrophages (BMMOs) were isolated from milk and seeded into culture dishes at 50,000 BMMOs/well. BMMOs were challenged with either heat-killed S. uberis strain <t>0140J</t> or SUB1154 deletion mutant (0140JΔ sub1154 ) at a multiplicity of infection (MOI) of 50:1 bacterium:BMMO and/or 2 nM rSUB1154 or rSUB1154NP (proteolytically compromised) protein. Supernatants were collected 20h after challenge and the concentration of IL-1β was measured by ELISA. BMMOs were unstimulated in the no treatment (NT) group and this mean was deducted from the other values, which were then standardised to the LPS (10 ng/mL) positive control. Data is presented as N=3 ± SD. Data was statistically analysed using a one-way ANOVA followed by Tukey multiple comparisons post hoc test (**** P< 0.0001).

S Uberis Strain 0140j, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Priming from within: TLR2 dependent but receptor independent activation of the mammary macrophage inflammasome by Streptococcus uberis"

Article Title: Priming from within: TLR2 dependent but receptor independent activation of the mammary macrophage inflammasome by Streptococcus uberis

Journal: Frontiers in Cellular and Infection Microbiology

doi: 10.3389/fcimb.2024.1444178

Figure Legend Snippet: S. uberis SUB1154 protein is involved in the production of IL-1β from BMMOs. Bovine mammary macrophages (BMMOs) were isolated from milk and seeded into culture dishes at 50,000 BMMOs/well. BMMOs were challenged with either heat-killed S. uberis strain 0140J or SUB1154 deletion mutant (0140JΔ sub1154 ) at a multiplicity of infection (MOI) of 50:1 bacterium:BMMO and/or 2 nM rSUB1154 or rSUB1154NP (proteolytically compromised) protein. Supernatants were collected 20h after challenge and the concentration of IL-1β was measured by ELISA. BMMOs were unstimulated in the no treatment (NT) group and this mean was deducted from the other values, which were then standardised to the LPS (10 ng/mL) positive control. Data is presented as N=3 ± SD. Data was statistically analysed using a one-way ANOVA followed by Tukey multiple comparisons post hoc test (**** P< 0.0001).

Techniques Used: Isolation, Mutagenesis, Infection, Concentration Assay, Enzyme-linked Immunosorbent Assay, Positive Control

S. uberis SUB1154 protein functions intracellularly to prime the NLRP3 inflammasome in BMMOs. Bovine mammary macrophages (BMMOs) were isolated from milk and seeded into culture dishes at 50,000 BMMOs/well. BMMOs were challenged with either heat-killed S. uberis strain 0140J or SUB1154 deletion mutant (0140JΔ sub1154 ) at a multiplicity of infection (MOI) of 50:1 bacterium:BMMO and/or 2 nM rSUB1154 protein. Cell entry was inhibited by incubating BMMOs with 10 µM Cytochalasin D (CyD) for 2h. Supernatants were collected 20h after challenge and the concentration of IL-1β was measured by ELISA. BMMOs were unstimulated in a no treatment group and the mean of this group was deducted from the other values and standardised to the LPS positive control (10 ng/mL). Data is presented as N=9 ± SD. Data was statistically analysed using a one-way ANOVA followed by Tukey multiple comparisons post hoc test ****=p<0.0001.

Figure Legend Snippet: S. uberis SUB1154 protein functions intracellularly to prime the NLRP3 inflammasome in BMMOs. Bovine mammary macrophages (BMMOs) were isolated from milk and seeded into culture dishes at 50,000 BMMOs/well. BMMOs were challenged with either heat-killed S. uberis strain 0140J or SUB1154 deletion mutant (0140JΔ sub1154 ) at a multiplicity of infection (MOI) of 50:1 bacterium:BMMO and/or 2 nM rSUB1154 protein. Cell entry was inhibited by incubating BMMOs with 10 µM Cytochalasin D (CyD) for 2h. Supernatants were collected 20h after challenge and the concentration of IL-1β was measured by ELISA. BMMOs were unstimulated in a no treatment group and the mean of this group was deducted from the other values and standardised to the LPS positive control (10 ng/mL). Data is presented as N=9 ± SD. Data was statistically analysed using a one-way ANOVA followed by Tukey multiple comparisons post hoc test ****=p<0.0001.

Techniques Used: Isolation, Mutagenesis, Infection, Concentration Assay, Enzyme-linked Immunosorbent Assay, Positive Control

SUB1154 primes the inflammasome by interacting with intracellular TIR domains. Bovine mammary macrophages (BMMOs) were isolated from milk and seeded into culture dishes at 50,000 BMMOs/well. BMMOs were incubated in the presence and absence of the TLR2 inhibitors C29 (intracellularly binds to the TIR (toll-interleukin receptor) domain; 100 µM) or MMG 11 (antagonist to extracellular binding of TLR2; 100 µM) 1h prior to subsequent challenge with either heat-killed S. uberis strain 0140J or SUB1154 deletion mutant (0140JΔ sub1154 ) at a multiplicity of infection (MOI) of 50:1 bacterium:BMMO and/or 2 nM rSUB1154 or rSUB1154NP (proteolytically compromised) protein; 1.0 µg/mL Pam3CSK4 (primes the inflammasome) and/or 500 µg/mL silica (activates the inflammasome). IL-1β concentration was measured from the supernatants after 20h by ELISA and values were standardised to LPS. Data is presented as N=3 ± SD, statistically analysed using a one-way ANOVA followed by Tukey multiple comparisons post hoc test ****=p<0.0001, ns, not significant, comparisons against 0140J alone. ND, none detected.

Figure Legend Snippet: SUB1154 primes the inflammasome by interacting with intracellular TIR domains. Bovine mammary macrophages (BMMOs) were isolated from milk and seeded into culture dishes at 50,000 BMMOs/well. BMMOs were incubated in the presence and absence of the TLR2 inhibitors C29 (intracellularly binds to the TIR (toll-interleukin receptor) domain; 100 µM) or MMG 11 (antagonist to extracellular binding of TLR2; 100 µM) 1h prior to subsequent challenge with either heat-killed S. uberis strain 0140J or SUB1154 deletion mutant (0140JΔ sub1154 ) at a multiplicity of infection (MOI) of 50:1 bacterium:BMMO and/or 2 nM rSUB1154 or rSUB1154NP (proteolytically compromised) protein; 1.0 µg/mL Pam3CSK4 (primes the inflammasome) and/or 500 µg/mL silica (activates the inflammasome). IL-1β concentration was measured from the supernatants after 20h by ELISA and values were standardised to LPS. Data is presented as N=3 ± SD, statistically analysed using a one-way ANOVA followed by Tukey multiple comparisons post hoc test ****=p<0.0001, ns, not significant, comparisons against 0140J alone. ND, none detected.

Techniques Used: Isolation, Incubation, Binding Assay, Mutagenesis, Infection, Concentration Assay, Enzyme-linked Immunosorbent Assay

S. uberis SUB1154 protein primes the NLRP3 inflammasome in BMMOs. Bovine mammary macrophages (BMMOs) at 50,000 BMMOs/well were challenged with heat-killed S. uberis strain 0140J or SUB1154 deletion mutant (0140JΔ sub1154 ) at a multiplicity of infection (MOI) of 50:1 bacterium:BMMO and/or 2 nM rSUB1154 or rSUB1154NP (mutant predicted protease site) protein. Supernatants were collected 20h after challenge and the concentration of IL-1β measured by ELISA. BMMOs were unstimulated in a no treatment group and the mean of this group was deducted from the other values and standardised to the LPS positive control (10 ng/mL). Data is presented as N=3 ± SD. In addition to S. uberis and rSUB1154 and NP proteins, BMMOs were challenged with 1.0 µg/mL Pam3CSK4 (primes the inflammasome) and 500 µg/mL silica (activates the inflammasome). Data was statistically analysed using a one-way ANOVA followed by Tukey multiple comparisons post hoc test (** P< 0.01 compared to silica + rSUB1154).

Figure Legend Snippet: S. uberis SUB1154 protein primes the NLRP3 inflammasome in BMMOs. Bovine mammary macrophages (BMMOs) at 50,000 BMMOs/well were challenged with heat-killed S. uberis strain 0140J or SUB1154 deletion mutant (0140JΔ sub1154 ) at a multiplicity of infection (MOI) of 50:1 bacterium:BMMO and/or 2 nM rSUB1154 or rSUB1154NP (mutant predicted protease site) protein. Supernatants were collected 20h after challenge and the concentration of IL-1β measured by ELISA. BMMOs were unstimulated in a no treatment group and the mean of this group was deducted from the other values and standardised to the LPS positive control (10 ng/mL). Data is presented as N=3 ± SD. In addition to S. uberis and rSUB1154 and NP proteins, BMMOs were challenged with 1.0 µg/mL Pam3CSK4 (primes the inflammasome) and 500 µg/mL silica (activates the inflammasome). Data was statistically analysed using a one-way ANOVA followed by Tukey multiple comparisons post hoc test (** P< 0.01 compared to silica + rSUB1154).

Techniques Used: Mutagenesis, Infection, Concentration Assay, Enzyme-linked Immunosorbent Assay, Positive Control

BMMO differential RNA expression of inflammasome pathway genes in response to S. uberis stimulation. RNA was extracted from isolated bovine mammary macrophages (BMMOs) at 0, 2, 4, 8, 12, 16 and 20h after challenge with either 10 ng/mL LPS; heat-killed S. uberis strain 0140J or SUB1154 deletion mutant (0140JΔ sub1154 ) at a multiplicity of infection (MOI) of 50:1 bacterium:BMMO; 2 nM rSUB1154 or rSUB1154NP (proteolytically compromised) protein. Changes in mRNA quantity were determined by real time reverse transcription quantitative PCR using 3 reference genes (GAPDH, ACTB and RPL13a) for 4 target genes: TLR2 (A) , NF-kB (B) , pro-caspase-1 (C) and pro-IL-1β (D) . Log 2 was calculated and the data is presented as N=3 ± SD. Values at 0h were used to determine baseline mRNA levels (0 Log 2 ). Asterisks denote significance calculated with Two-Way ANOVA and Dunnett’s multiple comparisons test, compared to 0140J, at 4h and 20h; * P< 0.05, ** P< 0.01,*** P< 0.001 **** P< 0.0001. All timepoint statistics available in <xref ref-type=

Supplementary Table 1 . " title="... either 10 ng/mL LPS; heat-killed S. uberis strain 0140J or SUB1154 deletion mutant (0140JΔ sub1154 ) at ..." property="contentUrl" width="100%" height="100%"/>
Figure Legend Snippet: BMMO differential RNA expression of inflammasome pathway genes in response to S. uberis stimulation. RNA was extracted from isolated bovine mammary macrophages (BMMOs) at 0, 2, 4, 8, 12, 16 and 20h after challenge with either 10 ng/mL LPS; heat-killed S. uberis strain 0140J or SUB1154 deletion mutant (0140JΔ sub1154 ) at a multiplicity of infection (MOI) of 50:1 bacterium:BMMO; 2 nM rSUB1154 or rSUB1154NP (proteolytically compromised) protein. Changes in mRNA quantity were determined by real time reverse transcription quantitative PCR using 3 reference genes (GAPDH, ACTB and RPL13a) for 4 target genes: TLR2 (A) , NF-kB (B) , pro-caspase-1 (C) and pro-IL-1β (D) . Log 2 was calculated and the data is presented as N=3 ± SD. Values at 0h were used to determine baseline mRNA levels (0 Log 2 ). Asterisks denote significance calculated with Two-Way ANOVA and Dunnett’s multiple comparisons test, compared to 0140J, at 4h and 20h; * P< 0.05, ** P< 0.01,*** P< 0.001 **** P< 0.0001. All timepoint statistics available in Supplementary Table 1 .

Techniques Used: RNA Expression, Isolation, Mutagenesis, Infection, Reverse Transcription, Real-time Polymerase Chain Reaction

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Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Priming from within: TLR2 dependent but receptor independent activation of the mammary macrophage inflammasome by Streptococcus uberis

doi: 10.3389/fcimb.2024.1444178

Figure Lengend Snippet: S. uberis SUB1154 protein is involved in the production of IL-1β from BMMOs. Bovine mammary macrophages (BMMOs) were isolated from milk and seeded into culture dishes at 50,000 BMMOs/well. BMMOs were challenged with either heat-killed S. uberis strain 0140J or SUB1154 deletion mutant (0140JΔ sub1154 ) at a multiplicity of infection (MOI) of 50:1 bacterium:BMMO and/or 2 nM rSUB1154 or rSUB1154NP (proteolytically compromised) protein. Supernatants were collected 20h after challenge and the concentration of IL-1β was measured by ELISA. BMMOs were unstimulated in the no treatment (NT) group and this mean was deducted from the other values, which were then standardised to the LPS (10 ng/mL) positive control. Data is presented as N=3 ± SD. Data was statistically analysed using a one-way ANOVA followed by Tukey multiple comparisons post hoc test (**** P< 0.0001).

Article Snippet: S. uberis strain 0140J (strain ATCC BAA-854/0140J), originally isolated from a clinical case of bovine mastitis in the UK, was used throughout this study as a reference strain.

Techniques: Isolation, Mutagenesis, Infection, Concentration Assay, Enzyme-linked Immunosorbent Assay, Positive Control

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Priming from within: TLR2 dependent but receptor independent activation of the mammary macrophage inflammasome by Streptococcus uberis

doi: 10.3389/fcimb.2024.1444178

Figure Lengend Snippet: S. uberis SUB1154 protein functions intracellularly to prime the NLRP3 inflammasome in BMMOs. Bovine mammary macrophages (BMMOs) were isolated from milk and seeded into culture dishes at 50,000 BMMOs/well. BMMOs were challenged with either heat-killed S. uberis strain 0140J or SUB1154 deletion mutant (0140JΔ sub1154 ) at a multiplicity of infection (MOI) of 50:1 bacterium:BMMO and/or 2 nM rSUB1154 protein. Cell entry was inhibited by incubating BMMOs with 10 µM Cytochalasin D (CyD) for 2h. Supernatants were collected 20h after challenge and the concentration of IL-1β was measured by ELISA. BMMOs were unstimulated in a no treatment group and the mean of this group was deducted from the other values and standardised to the LPS positive control (10 ng/mL). Data is presented as N=9 ± SD. Data was statistically analysed using a one-way ANOVA followed by Tukey multiple comparisons post hoc test ****=p<0.0001.

Article Snippet: S. uberis strain 0140J (strain ATCC BAA-854/0140J), originally isolated from a clinical case of bovine mastitis in the UK, was used throughout this study as a reference strain.

Techniques: Isolation, Mutagenesis, Infection, Concentration Assay, Enzyme-linked Immunosorbent Assay, Positive Control

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Priming from within: TLR2 dependent but receptor independent activation of the mammary macrophage inflammasome by Streptococcus uberis

doi: 10.3389/fcimb.2024.1444178

Figure Lengend Snippet: SUB1154 primes the inflammasome by interacting with intracellular TIR domains. Bovine mammary macrophages (BMMOs) were isolated from milk and seeded into culture dishes at 50,000 BMMOs/well. BMMOs were incubated in the presence and absence of the TLR2 inhibitors C29 (intracellularly binds to the TIR (toll-interleukin receptor) domain; 100 µM) or MMG 11 (antagonist to extracellular binding of TLR2; 100 µM) 1h prior to subsequent challenge with either heat-killed S. uberis strain 0140J or SUB1154 deletion mutant (0140JΔ sub1154 ) at a multiplicity of infection (MOI) of 50:1 bacterium:BMMO and/or 2 nM rSUB1154 or rSUB1154NP (proteolytically compromised) protein; 1.0 µg/mL Pam3CSK4 (primes the inflammasome) and/or 500 µg/mL silica (activates the inflammasome). IL-1β concentration was measured from the supernatants after 20h by ELISA and values were standardised to LPS. Data is presented as N=3 ± SD, statistically analysed using a one-way ANOVA followed by Tukey multiple comparisons post hoc test ****=p<0.0001, ns, not significant, comparisons against 0140J alone. ND, none detected.

Article Snippet: S. uberis strain 0140J (strain ATCC BAA-854/0140J), originally isolated from a clinical case of bovine mastitis in the UK, was used throughout this study as a reference strain.

Techniques: Isolation, Incubation, Binding Assay, Mutagenesis, Infection, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Priming from within: TLR2 dependent but receptor independent activation of the mammary macrophage inflammasome by Streptococcus uberis

doi: 10.3389/fcimb.2024.1444178

Figure Lengend Snippet: S. uberis SUB1154 protein primes the NLRP3 inflammasome in BMMOs. Bovine mammary macrophages (BMMOs) at 50,000 BMMOs/well were challenged with heat-killed S. uberis strain 0140J or SUB1154 deletion mutant (0140JΔ sub1154 ) at a multiplicity of infection (MOI) of 50:1 bacterium:BMMO and/or 2 nM rSUB1154 or rSUB1154NP (mutant predicted protease site) protein. Supernatants were collected 20h after challenge and the concentration of IL-1β measured by ELISA. BMMOs were unstimulated in a no treatment group and the mean of this group was deducted from the other values and standardised to the LPS positive control (10 ng/mL). Data is presented as N=3 ± SD. In addition to S. uberis and rSUB1154 and NP proteins, BMMOs were challenged with 1.0 µg/mL Pam3CSK4 (primes the inflammasome) and 500 µg/mL silica (activates the inflammasome). Data was statistically analysed using a one-way ANOVA followed by Tukey multiple comparisons post hoc test (** P< 0.01 compared to silica + rSUB1154).

Article Snippet: S. uberis strain 0140J (strain ATCC BAA-854/0140J), originally isolated from a clinical case of bovine mastitis in the UK, was used throughout this study as a reference strain.

Techniques: Mutagenesis, Infection, Concentration Assay, Enzyme-linked Immunosorbent Assay, Positive Control

Supplementary Table 1 . " width="100%" height="100%">

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Priming from within: TLR2 dependent but receptor independent activation of the mammary macrophage inflammasome by Streptococcus uberis

doi: 10.3389/fcimb.2024.1444178

Figure Lengend Snippet: BMMO differential RNA expression of inflammasome pathway genes in response to S. uberis stimulation. RNA was extracted from isolated bovine mammary macrophages (BMMOs) at 0, 2, 4, 8, 12, 16 and 20h after challenge with either 10 ng/mL LPS; heat-killed S. uberis strain 0140J or SUB1154 deletion mutant (0140JΔ sub1154 ) at a multiplicity of infection (MOI) of 50:1 bacterium:BMMO; 2 nM rSUB1154 or rSUB1154NP (proteolytically compromised) protein. Changes in mRNA quantity were determined by real time reverse transcription quantitative PCR using 3 reference genes (GAPDH, ACTB and RPL13a) for 4 target genes: TLR2 (A) , NF-kB (B) , pro-caspase-1 (C) and pro-IL-1β (D) . Log 2 was calculated and the data is presented as N=3 ± SD. Values at 0h were used to determine baseline mRNA levels (0 Log 2 ). Asterisks denote significance calculated with Two-Way ANOVA and Dunnett’s multiple comparisons test, compared to 0140J, at 4h and 20h; * P< 0.05, ** P< 0.01,*** P< 0.001 **** P< 0.0001. All timepoint statistics available in Supplementary Table 1 .

Article Snippet: S. uberis strain 0140J (strain ATCC BAA-854/0140J), originally isolated from a clinical case of bovine mastitis in the UK, was used throughout this study as a reference strain.

Techniques: RNA Expression, Isolation, Mutagenesis, Infection, Reverse Transcription, Real-time Polymerase Chain Reaction

Murine mammary gland response to inoculation with different strains of Streptococcus uberis. A Bacterial load, D IL-8 and E LCN2 concentrations in mouse mammary glands harvested 24 h after intraductally inoculating 10 3 CFU S. uberis 0140J or NADC C-1, as well as representative H&E-stained sections of B sham or C Streptococcus uberis 0140J-inoculated mammary glands at 24 h post-inoculation. No CFUs were retrieved from the S. uberis NCTC 3858 inoculated mouse mammary glands. Data are shown as individual points with a line indicating the mean and an error bar representing the SEM. A triple (***) asterisk indicates p < 0.001, whereas “ns” indicates non-significance corresponding to a p > 0.01. Pictures were taken at a 10 × and 40 × magnification, with scale bars indicating 100 µm and 25 µm, respectively.

Journal: Veterinary Research

Article Title: Priming of the murine mammary gland with Staphylococcus chromogenes IM reduces bacterial growth of Streptococcus uberis : a proof-of-concept study

doi: 10.1186/s13567-023-01156-y

Figure Lengend Snippet: Murine mammary gland response to inoculation with different strains of Streptococcus uberis. A Bacterial load, D IL-8 and E LCN2 concentrations in mouse mammary glands harvested 24 h after intraductally inoculating 10 3 CFU S. uberis 0140J or NADC C-1, as well as representative H&E-stained sections of B sham or C Streptococcus uberis 0140J-inoculated mammary glands at 24 h post-inoculation. No CFUs were retrieved from the S. uberis NCTC 3858 inoculated mouse mammary glands. Data are shown as individual points with a line indicating the mean and an error bar representing the SEM. A triple (***) asterisk indicates p < 0.001, whereas “ns” indicates non-significance corresponding to a p > 0.01. Pictures were taken at a 10 × and 40 × magnification, with scale bars indicating 100 µm and 25 µm, respectively.

Article Snippet: Three bovine-derived S. uberis ATCC strains, i.e., ATCC BAA-854 (a.k.a.

Techniques: Staining

Representation of the superchallenge experiment to investigate Staphylococcus chromogenes priming against Streptococcus uberis infection. Mice were intramammarily inoculated with either 10 4 CFU S. chromogenes IM (challenge) or sham (PBS), followed by a second inoculation 24 h later with either 10 3 CFU S. uberis NADC C-1 (infection) or sham (PBS). This led to 4 groups i.e., (1) S. chromogenes IM- S. uberis -inoculated (black), (2) S. chromogenes IM-sham (horizontal stripes), (3) sham- S. uberis (asterisks) and (4) sham-sham (vertical stripes) mice. “n” represents the number of animals inoculated in each group.

Journal: Veterinary Research

Article Title: Priming of the murine mammary gland with Staphylococcus chromogenes IM reduces bacterial growth of Streptococcus uberis : a proof-of-concept study

doi: 10.1186/s13567-023-01156-y

Figure Lengend Snippet: Representation of the superchallenge experiment to investigate Staphylococcus chromogenes priming against Streptococcus uberis infection. Mice were intramammarily inoculated with either 10 4 CFU S. chromogenes IM (challenge) or sham (PBS), followed by a second inoculation 24 h later with either 10 3 CFU S. uberis NADC C-1 (infection) or sham (PBS). This led to 4 groups i.e., (1) S. chromogenes IM- S. uberis -inoculated (black), (2) S. chromogenes IM-sham (horizontal stripes), (3) sham- S. uberis (asterisks) and (4) sham-sham (vertical stripes) mice. “n” represents the number of animals inoculated in each group.

Article Snippet: Three bovine-derived S. uberis ATCC strains, i.e., ATCC BAA-854 (a.k.a.

Techniques: Infection

Priming effect of Staphylococcus chromogenes IM in the murine mammary gland against Streptococcus uberis infection. Bacterial growth of A S. uberis and B S. chromogenes IM, concentrations of G IL-8 and H LCN2 in mouse mammary glands, as well as representative H&E-stained sections thereof, harvested 48 h after intraductally inoculating C , E sham (PBS) or D , F 10 4 CFU S. chromogenes IM, followed by a second inoculation after a 24 h interval with either C , D sham or E , F 10 3 CFU S. uberis NADC C-1. Data are shown as individual points with a line indicating the mean and an error bar representing the SEM. Double (**) and triple (***) asterisks indicate respectively p < 0.01 and p < 0.001, whereas “ns” indicates non-significance corresponding to a p > 0.05. Pictures were taken at a ×10 and ×40 magnification, with scale bars indicating 100 µm and 25 µm, respectively.

Journal: Veterinary Research

Article Title: Priming of the murine mammary gland with Staphylococcus chromogenes IM reduces bacterial growth of Streptococcus uberis : a proof-of-concept study

doi: 10.1186/s13567-023-01156-y

Figure Lengend Snippet: Priming effect of Staphylococcus chromogenes IM in the murine mammary gland against Streptococcus uberis infection. Bacterial growth of A S. uberis and B S. chromogenes IM, concentrations of G IL-8 and H LCN2 in mouse mammary glands, as well as representative H&E-stained sections thereof, harvested 48 h after intraductally inoculating C , E sham (PBS) or D , F 10 4 CFU S. chromogenes IM, followed by a second inoculation after a 24 h interval with either C , D sham or E , F 10 3 CFU S. uberis NADC C-1. Data are shown as individual points with a line indicating the mean and an error bar representing the SEM. Double (**) and triple (***) asterisks indicate respectively p < 0.01 and p < 0.001, whereas “ns” indicates non-significance corresponding to a p > 0.05. Pictures were taken at a ×10 and ×40 magnification, with scale bars indicating 100 µm and 25 µm, respectively.

Article Snippet: Three bovine-derived S. uberis ATCC strains, i.e., ATCC BAA-854 (a.k.a.

Techniques: Infection, Staining

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